目的 考察大株红景天片抗心肌缺血的药效作用及其相关作用机制。方法 采用冠脉结扎致大鼠心肌缺血模型,测定大株红景天片对缺血大鼠血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)及心肌组织B-raf、ras、p-ERK1/2蛋白的表达。结果 大株红景天片对冠脉结扎引起大鼠血清AST、ALT、 CK、CK-MB高表达有明显抑制作用,对冠脉结扎引起的大鼠心肌组织蛋白B-raf、Ras、p-ERK1/2表达有明显抑制作用,与模型组比较有显著性差异。结论 大株红景天片对大鼠冠脉结扎引起的心肌缺血有一定的对抗作用,其作用机制可能跟降低血清AST、ALT、 CK、CK-MB及心肌组织B-raf、ras、p-ERK1/2的高表达有关。
Abstract
OBJECTIVE To study the effect and action mechanism of the large plant Rhodiola Tablet on myocardial ischemia of rats. METHODS Rat myocardial ischemia models were induced by coronary artery ligation. The levels of AST, ALT, CK and CK-MB in serum were observed, at the same time the expressions of B-raf, Ras and p-ERK1/2 in myocardial tissue were determined. RESULTS The levels of AST, ALT, CK and CK-MB in rat serum, as well as the B-raf, Ras and p-ERK1/2 expression in rats myocardium in the large plant Rhodiola group were significantly lower than that in the model group. CONCLUSION Large plant Rhodiola has a certain antagonism on myocardial ischemia injury in rats caused by coronary artery ligation. The mechanism of action may be related to the decreases in serum AST, ALT, CK and CK-MB,as well as myocardial high expression of the B-raf, Ras and p-ERK1/2.
关键词
大株红景天片 /
氧化应激 /
心肌缺血
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Key words
large plant Rhodiola /
oxidative stress /
myocardial ischemia
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中图分类号：
R285.5
R965
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